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Magnetic separator
Šnédar, Jan ; Malášek, Jiří (referee) ; Jonák, Martin (advisor)
The aim of this bachelor thesis is to design the structural conception of belt magnetic separator with permanent magnet designated to separate unwanted ferromagnetic material waste from the machining of aluminium. This report contains an overview of equipment for the separation and design of concrete solutions to the magnetic separator. According to the input parameters are calculated all the parameters necessary for correct machine design. Attachments to this technical report are documentation drawings of selected parts and assemblies.
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Magnetic classification of fly ashes and application of products
Červený, Ľuboš ; Švec, Jiří (referee) ; Šoukal, František (advisor)
Submitted bachelor thesis is dealing with problematics of magnetic separation of fly ash and utilization of the products of the separation in production of refractory ceramic materials. High temperature fly ash from power station Mělník was used in this thesis.Thesis evaluates results of magnetic separation at different setting of magnetic separator. It proves, that fraction of particles rich in iron as well as fraction depleted by this iron can be obtained by magnetic separation. In this thesis are presented results of volumetric weight, mineralogic element composition, measurments by heating microscope and results of particle size distribution of unseparated fly ash, but also its both separates. The thesis is focusing on possibilities of utilization as additive in refractory ceramic materials and comparison of their refractory properties. It evaluates results of characterisation of the products using XRD analysis and scanning electron microscopy.
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Magnetic separator for recycling construction and demolition waste
Kovařík, Martin ; Zeizinger, Lukáš (referee) ; Škopán, Miroslav (advisor)
This work contains a search of available equipment for the separation of ferromagnetic material and the design of a specific design solution of a magnetic separator for the recycling of ferromagnetic waste from construction and demolition waste. According to the entered parameters, the calculations necessary for the correct design of the machine were performed. Part of the work is drawing documentation of selected parts and assemblies of the device.
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Nucleic acids isolation for diagnostic purposes using polymeric carriers
Syslová, Ivona ; Horák, Daniel (referee) ; Rittich, Bohuslav (advisor)
The isolation of deoxyribonucleic acid (DNA) was studied in the diploma thesis by using three different methods: phenol extraction, salting with sodium chloride and magnetic separation with reversible adsorption of nucleic acids on different magnetic carriers. There were used five different properly functionalized carriers for the isolation of DNA: magnetic silicagel, P(HEMA-co-GMA) ox. I, P(HEMA-co-GMA) ox. II, Dynal DNA Direct and Perovskit 439. The reversible imobilization of DNA on the magnetic carrier was proceeded under the conditions of high concentration of NaCl and poly(ethyleneglycol) (PEG). There was induced the condensation of DNA by 2 M NaCl and PEG with molecular mass 6000 for binding of the DNA to the magnetic carriers and the final concentration of PEG in the separation mixture was 8 and 16 %. The aim was to gain the DNA of quality suitable for polymerase chain reaction (PCR). The DNA was isolated from the bacterial cultures of three probiotic strains, L. amylovorus CCM 4380T, L. zeae CCM 7069 T, L. plantarum CCM 7039T, which were cultivated in MRS medium. The DNA was also isolated from the fermented dairy products: Jihočeský zákys s ovocem jahoda (the fermented dairy product with the probiotic culture of Lactobacillus acidophilus, Bifidobacterium lactis and Streptococcus thermophilus), Revital active (the yogurt with inulin and the probiotic culture of Lactobacillus rhamnosus, Lactobacillus acidophilus and Bifidobacterium sp.) and Actimel višeň (the dairy product with the probiotic culture of Lactobacillus casei). When the PCR with the isolated DNA was passed off, the PCR products were detected by the gel electrophoresis with agarose. The success of the DNA isolation of the probiotic bacteria by phenol extraction, salting with NaCl and by magnetic separation, was verified by the PCR method. The method of magnetic separation using magnetic carriers was also verified for the isolation of DNA of quality suitable for PCR from the probiotic fermented dairy products.
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Magnetic separator
Křupka, Jiří ; Jonák, Martin (referee) ; Malášek, Jiří (advisor)
The aim of this bachelor thesis is to design the structural conception of magnetic separator designated to separate small-sized industrial waste. This report provides an overview of facilities of worldwide manufacturers that are used for magnetic separation of steel scrap. Furthermore, it offers an assessment of variants for technical solutions to structural nodes of magnetic separator; this is followed by a selection of specific solution. According to the input parameters, all the parameters necessary for correct machine design are computed. These computations also include the justification of selected table values, placement, surface roughness, geometric tolerances, materials and heat treatment of the individual parts and assemblies. Attachments to this technical report are documentation drawings of selected parts and assemblies.
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Magnetic separator for recycling construction and demolition waste
Kovařík, Martin ; Zeizinger, Lukáš (referee) ; Škopán, Miroslav (advisor)
This work contains a search of available equipment for the separation of ferromagnetic material and the design of a specific design solution of a magnetic separator for the recycling of ferromagnetic waste from construction and demolition waste. According to the entered parameters, the calculations necessary for the correct design of the machine were performed. Part of the work is drawing documentation of selected parts and assemblies of the device.
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Progresivní nástroje pro identifikaci bakterie Staphylococcus aureus
Číhalová, Kristýna
The presence of infections caused by Staphylococcus aureus (S. aureus) and its resistant form - methicillin-resistant S. aureus (MRSA) - are more frequent and treatment with increasing resistance becomes increasingly complicated. Early identification of microbiome in the infectious wounds leads to implementation of the correct treatment and thereby saving the patient from the formation of abscesses, amputation of limbs or even death. Presented thesis entitled Progressive tools for the identification of Staphylococcus aureus is focused on the study of the occurrence of these bacteria in clinical samples, design and construction of new detection methods based on magnetic separation and evaluation of the effects of antibiotics and metal nanoparticles on growth and biofilm formation, gene expression and protein composition in S. aureus and MRSA. The most abundant microorganism in microbiome of infections is S. aureus and in our studies was used as model bacteria for the design of fast, accurate and sensitive detection techniques utilizing indirect detection such as: i) detection of product of biochemical reaction between bacterial enzymes and matrix, ii) detection of the amplified gene using gold nanoparticles as an indicator probe, iii) determination of the detection oligonucleotides after sandwich capture of bacteria between micro and nanoparticles, and iv) a multiplex detection of quantum dots using sandwich capture of specific gene from selected bacteria. Subsequently, an influence effect of beta-lactam antibiotics and their complexes with selenium nanoparticles to non-resistant and resistant form of S. aureus was investigated. The significant inhibition of bacterial growth and biofilm formation as well as changes in gene expression and protein composition were achieved in MRSA by using the complexes of antibiotics and selenium nanoparticles.
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Magnetic classification of fly ashes and application of products
Červený, Ľuboš ; Švec, Jiří (referee) ; Šoukal, František (advisor)
Submitted bachelor thesis is dealing with problematics of magnetic separation of fly ash and utilization of the products of the separation in production of refractory ceramic materials. High temperature fly ash from power station Mělník was used in this thesis.Thesis evaluates results of magnetic separation at different setting of magnetic separator. It proves, that fraction of particles rich in iron as well as fraction depleted by this iron can be obtained by magnetic separation. In this thesis are presented results of volumetric weight, mineralogic element composition, measurments by heating microscope and results of particle size distribution of unseparated fly ash, but also its both separates. The thesis is focusing on possibilities of utilization as additive in refractory ceramic materials and comparison of their refractory properties. It evaluates results of characterisation of the products using XRD analysis and scanning electron microscopy.
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Magnetic biocomposite materials for removal of significant xenobiotics from water systems
BALDÍKOVÁ, Eva
The theoretical part of this doctoral thesis provides a comprehensive overview on the topic of preparation and subsequent utilization of magnetic derivatives of biological materials for xenobiotic separation from water. Main attention is paid to magnetic modification of waste materials and by-products originating from agricultural and food industry, which represent widely available and low-cost materials, and also to magnetic modification of microbial cells. In addition to the description of magnetic particle preparation and individual developed techniques of magnetic modification, a brief characterization of selected pollutants and a detailed table overview on utilization of magnetically responsive biomaterials for biosorption of organic dyes, heavy metals, pharmaceutical and personal care products together with ubiquitous industrial endocrine disruptors and also of crude oil derivatives is presented. Experimental part of this thesis is focused on the preparation and optimization of new types of magnetic materials. Emphasis is placed on the employment of simple, fast and simultaneously low-cost magnetic modification techniques (e.g., postmagnetization using microwave-synthesized magnetic iron oxides or one-step modification by magnetic fluids). Selected plant materials (barley and rye straw) were chemically modified to significantly (up to five-times) increase the maximum adsorption capacities for tested dyes. All prepared biomaterials exhibited a great magnetic response and simultaneously relatively high adsorption capacity for selected xenobiotics under experimental conditions used. Factors substantially affecting adsorption process, such as pH, initial concentration, incubation time or temperature were also studied. Adsorption equilibrium data were assessed using Langmuir, Freundlich or Sips isotherm models. Experimental data from time dependence study were analyzed by chosen kinetic models, namely the pseudo-first-order and pseudo-second-order ones and by intraparticle diffusion model. Thermodynamic parameters (Gibbs free energy, enthalpy and entropy) describing the nature of adsorption were also included in study.
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Magnetic separator
Šnédar, Jan ; Malášek, Jiří (referee) ; Jonák, Martin (advisor)
The aim of this bachelor thesis is to design the structural conception of belt magnetic separator with permanent magnet designated to separate unwanted ferromagnetic material waste from the machining of aluminium. This report contains an overview of equipment for the separation and design of concrete solutions to the magnetic separator. According to the input parameters are calculated all the parameters necessary for correct machine design. Attachments to this technical report are documentation drawings of selected parts and assemblies.
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